Laboratory for Development of Molecular Diagnostic Approaches



Candidate of Biological Sciences

Specialist in the field of molecular biology, chemical synthesis and modification of oligonucleotides, development of molecular diagnostics tools. Author of more than 25 publications and 3 patents. In 2020, he was awarded the medal ” Халық алғысы”(«Gratitude of the nation»).

ORCID ID – 0000-0002-6219-4002

Scopus ID – 57367964400


  • Synthesis of primers;
  • Synthesis of oligomers;
  • Synthesis of ultra-long oligomers;
  • Modification of oligonucleotides (5’ labeling with fluorescent labels);
  • De novo gene synthesis;
  • Development of kits for SNP analysis by Real-time PCR;
  • Development of kits for the identification of infectious diseases.


  • Innovation patent number: 30796, published: 25.12.2015. A method for identifying potato varieties and forms based on the use of microsatellite markers.
  • Innovation patent number: 29151, published: 17.11.2014. The genetic construct of pET28c is for the expression of the recombinant protein CagA Helicobacter pylori.
  • Patent number: 28630, published: 16.06.2014. Express method of Warfarin dose selection based on polymerase chain reaction in real time.

Core publications

Perfilyeva Y., Berdygulova Z., Mashzhan A., Zhigailov A., Ostapchuk Y., Naizabayeva D., Cherusheva A., Bissenbay A., Kuatbekova S., Abdolla N., Nizkorodova A., Kulemin M., Shapiyeva Z., Sayakova Z., Perfilyeva A., Akhmetollayev I., Maltseva E., Skiba Y.A, Mamadaliyev S., Dmitrovskiy A.
Molecular and seroepidemiological investigation of Сoxiella burnetii and spotted fever group rickettsiae in the southern region of Kazakhstan // Ticks Tick Borne Dis. 2023 Nov;14(6):102240. doi: 10.1016/j.ttbdis.2023.102240. Epub 2023 Aug 28. Процентиль – 77, рейтинг журнала по WOS – Q2, IF-3,2.

Kuibagarov M., Makhamed R., Zhylkibayev A., Berdikulov M., Abdrakhmanov S., Kozhabayev M., Akhmetollayev I., Mukanov K., Ryskeldina A., Ramankulov Y., Shustov A., Bauer C., Shevtsov A.
Theileria and Babesia infection in cattle - First molecular survey in Kazakhstan // Ticks Tick Borne Dis. 2023 Jan;14(1):102078. PMID: 36395616 DOI: 10.1016/j.ttbdis.2022.102078, Процентиль – 77, рейтинг журнала по WOS – Q2, IF-3,2.

Kanayev D., Abilmazhenova D., Akhmetollayev I., Sekenova A., Ogay V., Kulyyassov A.
Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR // Life 2023, 13(1), 107; PMID: 36676054 PMCID: PMC9862934 DOI: 10.3390/life13010107. Процентиль – 54, рейтинг журнала по WOS – Q2, IF-3,2.

Kalendar R., Shustov A., Akhmetollayev I., Kairov U.
Designing Allele-Specific Competitive-Extension PCR-Based Assays for High-Throughput Genotyping and Gene Characterization // Front Mol Biosci. 2022 Mar 1;9:773956. PMID: 35300118 PMCID: PMC8921500 DOI: 10.3389/fmolb.2022.773956. Процентиль – 70, рейтинг журнала по WOS – Q2, IF-5.

Mukhtarova K., Zholdybayeva E., Kairov U., Akhmetollayev I., Nurimanov C., Kulmirzayev M., Makhambetov Y., Ramankulov Y.
Whole-Exome Sequencing Reveals Pathogenic SIRT1 Variant in Brain Arteriovenous Malformation: A Case Report // Genes (Basel). 2022 Sep 21;13(10):1689. PMID: 36292575 PMCID: PMC9601721 DOI: 10.3390/genes13101689, Процентиль – 63, рейтинг журнала по WOS – Q2, IF-3,5.

Zhigailov A., Maltseva E., Perfilyeva Y., Ostapchuk Y., Naizabayeva D., Berdygulova Z., Kuatbekova S., Nizkorodova A., Mashzhan A., Gavrilov A., Abayev A., Akhmetollayev I., Mamadaliyev S., Skiba Y.
Prevalence and genetic diversity of coronaviruses, astroviruses and paramyxoviruses in wild birds in southeastern Kazakhstan // Heliyon. 2022 Oct 31;8(11):e11324. PMID: 36353173 PMCID: PMC9638769 DOI: 10.1016/j.heliyon.2022.e11324, Процентиль – 70, рейтинг журнала по WOS – Q2, IF-4.

Aitkulova A., Mukhtarova K., Zholdybayeva E., Medetov Y., Dzhamantayeva B., Kassymbek K., Utupov T., Akhmetollayev I., Akshulakov S., Kulmambetova G., Ramankulov Y.
Activated leukocyte cell adhesion molecule/cluster of differentiation 166 rs10933819 (G>A) variant is associated with familial intracranial aneurysms // Biomed Rep. 2022 Jun 7;17(2):65., PMID: 35815187 PMCID: PMC9260160 DOI: 10.3892/br.2022.1548, Процентиль – 71, рейтинг журнала по WOS – Q3, IF-2.3.

Tarlykov P., Atavliyeva S., Auganova D., Akhmetollayev I., Loshakova T., Varfolomeev V., Ramankulov Y.
Mitochondrial DNA analysis of ancient sheep from Kazakhstan: evidence for early sheep introduction // Heliyon. 2021 Sep 16;7(9):e08011., PMID: 34585018 PMCID: PMC8453193 DOI: 10.1016/j.heliyon.2021.e08011, Процентиль – 70, рейтинг журнала по WOS – Q2, IF-4.