Relevance

Widespread pesticides application in many agricultural systems, including Kazakhstan, resulted in soil and ground water contamination, posing serious health and environmental risks. Bioremediation, in which pesticide-degrading bacteria play a central role is considered as suitable, low-cost, and effective solution to clean up the polluted substrates. Besides bioremediation, bacteria can be applied to promote plant growth, protect from plant pathogens, sustain and increase soil fertility.

Aims

The project’s aim is isolation, identification, characterisation, and application of pesticide-degrading bacteria with plant-growth-promoting potential. The obtained results will broaden the collection of beneficial bacteria for utilisation in agricultural systems, providing effective solutions for cleaning up the polluted soils and stimulating plant growth in Kazakhstani agriculture.

Expected results

Endemic pesticide-degrading bacterial strains will be isolated from the crop rhizosphere, potting soil, and field soil samples. The isolates will be identified using their genetic sequence and any human or plant pathogens and potentially hazardous isolates will be excluded. The pesticide-degrading potential of the strains will be evaluated and the most effective strains will be selected. Pesticide biodegradation will be optimised via adjustments to eco-physiological conditions and by employing response surface methodology. The most promising isolates will be selected based on their pesticide-degrading potential.

Project supervisor

Zhumakayev A.R. H-index: 3, ResearcherID: GZG-9689-2022,

ORCID: 0000-0001-9022-0741, Scopus Author ID: 57193544703

Research group members

Abeldenov S.K. H-index – 4, ResearcherID F-5139-2015,

ORCID 0000-0002-6974-9138, Scopus Author ID 56674705400

SattarovaA.K. ORCID 0009-0006-3841-2467

Zhaksybek Zh. Zh. ORCID 0009-0008-2385-6449

Publications and security documents of the project supervisor and the research group related to the topic of the project

1. Zhumakayev, A.R., Varga, Vörös, Kocsubé, S., Ramteke, Szekeres,, Vágvölgyi, Hatvani, Marik (2022) Characterization of the antagonistic potential of the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 strain against the plant pathogenic bacterium Agrobacterium tumefaciens. Frontiers in Plant Science, section Plant Symbiotic Interactions. doi: 10.3389/fpls.2022.1034237
2. Allaga, H., Zhumakayev, A., Büchner., Kocsubé, Szűcs, Vágvölgyi, Kredics, Hatvani (2021) Members of the Trichoderma harzianum species complex with mushroom pathogenic potential. Agronomy, https://doi.org/10.3390/agronomy11122434
3. Zhumakayev, A. R., Vörös, Szekeres, Rakk, Vágvölgyi, Szűcs, Kredics, Škrbić, Hatvani (2021). Comprehensive characterization of stress tolerant bacteria with plant growth-promoting potential isolated from glyphosate-treated environment. World Journal of Microbiology & Biotechnology, https://doi.org/10.1007/s11274-021-03065-8

Achieved results

Endemic pesticide-degrading bacterial strains were isolated from the crop rhizosphere, potting soil, and field soil samples. The isolates were identified using their genetic sequence and any human or plant pathogens and potentially hazardous isolates were excluded. The pesticide-degrading potential of the strains was evaluated and the most effective strains were selected. Pesticide biodegradation was optimised via adjustments to eco-physiological conditions and by employing response surface methodology. The most promising isolates were selected based on their pesticide-degrading potential.