Atherosclerosis (AS) is a common vascular disease, which is based on the presence of lipid plaques in the vascular endothelium, with consequences associated with insufficient blood supply to organs and tissues. This disease affects 17.8% of the population in the world and 22.4% in Kazakhstan. Premature mortality of the population and consequences in the form of cardiovascular diseases, as well as early disability necessitate the search for new approaches to its treatment. Many studies in preclinical models of atherosclerosis demonstrate the significant potential of mesenchymal stem cells (MSCs) in inhibiting the development of atherosclerosis and their immunosuppressive, regenerative and atheroprotective effect of MSCs on atherosclerotic lesions of the vascular endothelium. The use of exosomes isolated from mesenchymal stem cells from various sources has significant potential in the treatment of atherosclerosis.
The goal of the project is to investigate the effect of exosomes isolated from cytokine-preconditioned human umbilical cord mesenchymal stem cells on an experimental animal with induced atherosclerosis.
1. The culture of human umbilical cord MSCs will be isolated and characterized.
2. Exosomes from human umbilical cord MSCs (hUC-MSCs) will be isolated and characterized.
3. The optimal cytokines and preconditioning period for effectively enhancing the immunomodulatory properties of hUC-MSCs and their exosomes in vitro will be determined.
4. In vivo studies will be conducted on a mouse model with induced atherosclerosis to determine the therapeutic effect of exosomes from cytokine-preconditioned hUC-MSCs.
Sekenova Aliya – Scopus ID – 57218821100