Laboratory of Genetic Engineering

Head of the laboratory



Candidate of Biology Sciences 


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Research Associate, Department of Microbiology, University of Alabama (USA), Department of Microbiology and Immunology, Medical Department of the University of Texas (USA);

Research scientist, State Research Center of Virology and Biotechnology

Fields of professional interest: genetic engineering of microorganisms, virology, bioengineering of expression systems for use in mammalian cells or in bacteria, development of processes for purification of recombinant proteins. Over the last 5 years supervised projects on the development of expression systems based on autonomously replicating viral genomes, development of novel antigens in the form of virus-like particles.

Research directionsCore publicationsInnovative developmentsServices
  • Development of technology for production of recombinant proteins with pharmacological activity (erythropoietin, antihemophilic factor VIII) in cultured mammalian cells using viral replicons as vectors;
  • Bioengineering of new antigens based on virus-like particles;
  • Production of recombinant antigens and enzymes in bacterial expression systems.
  • Foy N.J., Akhrymuk M., Shustov A.V., Frolova E.I., Frolov I. 

    Hypervariable Domain of Nonstructural Protein nsP3 of Venezuelan Equine Encephalitis Virus Determines Cell-Specific Mode of Virus Replication. J Virol. 2013 Jul;87(13):7569-84.

  • Shustov A.V., Frolov I.

    Efficient, trans-complementing packaging systems for chimeric, pseudoinfectious dengue 2/yellow fever viruses. J Virol. 2010 Apr 25;400(1):8-17.

  • McGee C.E., Shustov A.V., Tsetsarkin K., Frolov I.V., Mason P.W., Vanlandingham D.L.

    Higgs S.Infection, Dissemination, and Transmission of a West Nile Virus Green Fluorescent Protein 4nfectious Clone by Culex pipiens quinquefasciatus mosquitoes. Vector Borne Zoonotic Dis. 2010 Apr;10(3):267-74.

  • Kamzina A.S., Baltabekova A. Zh., Shustov A.V.

    Virus-like particles presenting conformational epitopes as a promising vaccine platform. Биотехнология: теория и практика. 2012; 4:3-9.

  • Ахметова Д.Г., Бердыгулова Ж.А., Евтыхова Е.Б., Шустов А.В.

    Сальмонеллы молекулярные механизмы приспособленности и факторы вирулентности. Биотехнология: теория и практика. 2012; 1:3-24.

  • Евтыхова Е.Б., Силаев Д.В., Балтабекова А.Ж., Шустов А.В.

    Термостабильная рекомбинантная фитаза, произведенная в E. coli. Биотехнология: теория и практика. 2014; 1:62-70.

  • Патент США:

    (WO/2008/137163) TWO-COMPONENT GENOME FLAVIVIRUS AND USES THEREOF. Inventors: Ilya V. Frolov and Alexandr V. Shustov. Houston TX US.Pub. No.: WO/2008/137163 International Application No.: PCT/US2008/005824. Publication Date: 13.11.2008 International Filing Date: 07.05.2008 Patent application number: 20090324623. Patent applications by ADLER, Benjamin, Aaron; Adler & Associates, 8011 Candle Ln, Houston, TX 77071 (US).

  • Synthesis of DNA oligonucleotides (6-60 nt);
  • Production of synthetic genes and DNA fragments (de novo synthesis of genes with sequences provided by customer), with lengths 100 bp - 3000 bp;
  • Designing the expression constructs for customers and assembly of constructs using genetic engineering. Production of constructs (plasmids, replicons) for expression of the recombinant proteins in cultured mammalian cells or in E.coli, and development of customized expression and purification technology;
  • Production of recombinant proteins in cultured mammalian cells or in E.coli.

About us

Republican State Enterprise “National Center for Biotechnology” under the Science Committee of Ministry of Education and Science of the Republic of Kazakhstan (hereinafter – NCB) - country’s leading biological center,  implementing the State policy on support and development of biotechnology industry – was founded by the Decree of the President of the Republic of Kazakhstan in 1993. The center implements and coordinates the government-funded scientific – technical programmes in the field of biotechnology, biosafety and ecology.

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